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3525-C BINGE ALCOHOL INTAKE IN OBESITY INCREASES SERUM AND HEPATIC ETHYL ARACHIDONATE AND EXACERBATES LIVER INJURY Kumiko Shirai 1 , Hayato Hikita 2 , Sadatsugu Sakane 3 , Kazuhiro Murai 1 , Akira Nishio 4 , Kunimaro Furuta 1 , Takahiro Kodama 5 , Tomohide Tatsumi 5 and Tetsuo Takehara 5 , (1)Osaka University, Graduate School of Medicine, (2)Osaka University, Graduate School of Medicine, Osaka, Japan, (3)Osaka National Hospital, San Diego, CA, (4)Osaka University Graduate School of Medicine, Bethesda, MD, (5)Osaka University Graduate School of Medicine Background: Obesity and alcohol consumption work synergistically to exacerbate liver injury

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Other studies support that carotenoids can reduce the risk of cardiovascular complications by lowering blood pressure, reducing pro-inflammatory cytokines and markers of inflammation (like C-reactive protein), and improving insulin sensitivity in muscle, liver, and adipose tissues 14

Ex vivo reactivation of PBMCs Short-term reactivation of cryopreserved PBMCs and subsequent CyTOF were performed as previously described 99